2017 ( 8 )

2016 ( 18 )

2015 ( 289 )

2014 ( 409 )


匹配条件: “ Oliver Puk” ,找到相关结果约4627条。
Language Strategy in a Merger Process
Puk Lundgaard S?rensen
Language at Work : Bridging Theory and Practice , 2012,
Abstract: There is no point in having a language strategy which merely collects dust on the shelf because it is out of touch with the reality in the company. The strategy must be used in everyday work so that it becomes a proper management tool. If Energinet.dk succeeds in doing so, the company will be able to use the language strategy to unite the company, with a concern to language and achieve a high qualitative level as well.
The Pathologic Effect of a Novel Neomorphic Fgf9Y162C Allele Is Restricted to Decreased Vision and Retarded Lens Growth
Oliver Puk, Gabriele M?ller, Arie Geerlof, Kathrin Krowiorz, Nafees Ahmad, Sibylle Wagner, Jerzy Adamski, Martin Hrabé de Angelis, Jochen Graw
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0023678
Abstract: Fibroblast growth factor (Fgf) signalling plays a crucial role in many developmental processes. Among the Fgf pathway ligands, Fgf9 (UniProt: P54130) has been demonstrated to participate in maturation of various organs and tissues including skeleton, testes, lung, heart, and eye. Here we establish a novel Fgf9 allele, discovered in a dominant N-ethyl-N-nitrosourea (ENU) screen for eye-size abnormalities using the optical low coherence interferometry technique. The underlying mouse mutant line Aca12 was originally identified because of its significantly reduced lens thickness. Linkage studies located Aca12 to chromosome 14 within a 3.6 Mb spanning interval containing the positional candidate genes Fgf9 (MGI: 104723), Gja3 (MGI: 95714), and Ift88 (MGI: 98715). While no sequence differences were found in Gja3 and Ift88, we identified an A→G missense mutation at cDNA position 770 of the Fgf9 gene leading to an Y162C amino acid exchange. In contrast to previously described Fgf9 mutants, Fgf9Y162C carriers were fully viable and did not reveal reduced body-size, male-to-female sexual reversal or skeletal malformations. The histological analysis of the retina as well as its basic functional characterization by electroretinography (ERG) did not show any abnormality. However, the analysis of head-tracking response of the Fgf9Y162C mutants in a virtual drum indicated a gene-dosage dependent vision loss of almost 50%. The smaller lenses in Fgf9Y162C suggested a role of Fgf9 during lens development. Histological investigations showed that lens growth retardation starts during embryogenesis and continues after birth. Young Fgf9Y162C lenses remained transparent but developed age-related cataracts. Taken together, Fgf9Y162C is a novel neomorphic allele that initiates microphakia and reduced vision without effects on organs and tissues outside the eye. Our data point to a role of Fgf9 signalling in primary and secondary lens fiber cell growth. The results underline the importance of allelic series to fully understand multiple functions of a gene.
Pleiotropic effects in Eya3 knockout mice
Torben S?ker, Claudia Dalke, Oliver Puk, Thomas Floss, Lore Becker, Ines Bolle, Jack Favor, Wolfgang Hans, Sabine M H?lter, Marion Horsch, Magdalena Kallnik, Eva Kling, Corinna Moerth, Anja Schrewe, Christian Stigloher, Stefanie Topp, Valerie Gailus-Durner, Beatrix Naton, Johannes Beckers, Helmut Fuchs, Boris Ivandic, Thomas Klopstock, Holger Schulz, Eckhard Wolf, Wolfgang Wurst, Laure Bally-Cuif, Martin de Angelis, Jochen Graw
BMC Developmental Biology , 2008, DOI: 10.1186/1471-213x-8-118
Abstract: Expression analysis of Eya3 by in-situ hybridizations and β-Gal-staining of Eya3 mutant mice revealed abundant expression of the gene throughout development, e.g. in brain, eyes, heart, somites and limbs suggesting pleiotropic effects of the mutated gene. A similar complex expression pattern was observed also in zebrafish embryos.The phenotype of young adult Eya3 mouse mutants was systematically analyzed within the German Mouse Clinic. There was no obvious defect in the eyes, ears and kidneys of Eya3 mutant mice. Homozygous mutants displayed decreased bone mineral content and shorter body length. In the lung, the tidal volume at rest was decreased, and electrocardiography showed increased JT- and PQ intervals as well as decreased QRS amplitude. Behavioral analysis of the mutants demonstrated a mild increase in exploratory behavior, but decreased locomotor activity and reduced muscle strength. Analysis of differential gene expression revealed 110 regulated genes in heart and brain. Using real-time PCR, we confirmed Nup155 being down regulated in both organs.The loss of Eya3 in the mouse has no apparent effect on eye development. The wide-spread expression of Eya3 in mouse and zebrafish embryos is in contrast to the restricted expression pattern in Xenopus embryos. The loss of Eya3 in mice leads to a broad spectrum of minor physiological changes. Among them, the mutant mice move less than the wild-type mice and, together with the effects on respiratory, muscle and heart function, the mutation might lead to more severe effects when the mice become older. Therefore, future investigations of Eya3 function should focus on aging mice.Eya3 is one of four mammalian orthologous genes (Eya1-4) of eyes absent (eya) in Drosophila melanogaster [1,2]. Previous investigations demonstrated that a homozygous knockout of eya function in D. melanogaster results in severe embryonic defects and absence of compound eyes due to eye progenitor cell death [3,4]. Like eyes absent in Drosophil
Eva Dudriková,Lucia Po?aková,Jana Puká?ová
Potravinarstvo : Scientific Journal for Food Industry , 2010, DOI: 10.5219/24
Abstract: Totally, 47 strains of S. aureus and 578 coagulase negative staphylococci were detected in samples from raw ewe milk. The 35 out 47 isolates of S. aureus from ewe milk were positive for the presence of staphylococcal enterotoxin genes: sea(4 %), sec (48 %) a sed (48 %). Staphylococcus epidermis (33.04%), Staphylococcus caprae (21.28%) were more prevalent. Staphylococcus chromogenes (7.44 %), Staphylococcus hominis (7.09%), Staphylococcus xylosus (6,92 %), a Staphylococcus warneri (6.40 %) were isolated also in ewes milk. Staphylococcus haemolyticus (3.11 %), Staphylococcus capitis (2.94 %), Staphylococcus simulans (2.08 %) and Staphylococcus saprophyticus (1.73 %) were isolated very rarely from the taken individual milk ewe samples. Sporadically, only in few cases, the others coagulase negative staphylococci were isolated (< 1 %): Staphylococcus cohnii cohnii, Staphylococcus sciuri, Staphylococcus closii, Staphylococcus lugdunensis, Staphylococcus auricularis and Staphylococcus equorum. doi:10.5219/24
Jana Puká?ová,Lucia Po?aková,Eva Dudriková
Potravinarstvo : Scientific Journal for Food Industry , 2010, DOI: 10.5219/21
Abstract: Strains of S. aureus were isolated from individual milk samples of 500 lacting cows from different areas in Slovakia (PH 1 - Ko ice region, 300 samples; PH 2 - ilina region, 200 samples). The statistical significance between both dairy farms included in the experiment in the presence of genus Staphylococcus isolated on Baird-Parker agar from milk samples was p < 0,0001(***) . Totally, 122 milk samples were positive for the presence of Staphylococcus aureus (83 Ko ice regions, 52 ilina regions). All 122 isolates of S. aureus were sensitive for the methicilin as detected by interpretative criteria developed by NCCLS (2002). For 122 S. aureus isolates, we compared antibiotic susceptibility results determined by the standardized agar diffusion assay with the PCR assay for the detection of antibiotic resistance mecA gene. For all isolates, we found a correlation between the results of the PCR and those of classical resistance testing. The obtained results were confirmed by PCR analysis, according to which, any of our tested isolate of S. aureus from all 122 individual milk samples from both experimental dairy farms were not positive for the presence of mecA gene coding the methicilin resistance. doi:10.5219/21
Cervical Stiffness Evaluated In Vivo by Endoflip in Pregnant Women
Lene Hee, Donghua Liao, Puk Sandager, Hans Gregersen, Niels Uldbjerg
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0091121
Abstract: Objective To determine the stiffness of the pregnant uterine cervix in vivo. Method Five women in early pregnancy and six women in late pregnancy were included. The EndoFlip is a 1-m-long probe with a 12-cm-long bag mounted on the tip. The tip of the probe was inserted into the cervical canal. Sensors spaced at 0.5-cm intervals along the probe were used to determine 16 serial cross-sectional areas of the bag. The diameter of the cervical canal could thereby be determined during inflation with up to 50 ml saline solution. Tissue stiffness was calculated from the geometric profiles and the pressure-strain elastic modulus (EP) at each sensor site. Three parts of the cervix were defined: the uterus-near part, the middle and the vaginal part. The EPmax was defined as the highest EP detected along the cervical canal. Results The EPmax was always found in the middle part of the cervix. The median EPmax was 243 kPa (IQR, 67–422 kPa) for the early pregnant women and 5 kPa (IQR, 4–15 kPa) for those at term. In the early pregnant women the stiffness differed along the cervical length (p<0.05) whereas difference along the cervix was not found for late pregnant women. A positive correlation coefficient (Spearman’s rho) was established between the EPs of the uterus-near and the middle part (0.84), between the vaginal and the middle part (0.81), and between the uterus-near and the vaginal part (0.85). Conclusion This new method can estimate the stiffness along the cervical canal in vivo. This method may be useful in the clinical examination of the biomechanical properties of the uterine cervix.
Renewing Oncological Hyperthermia—Oncothermia  [PDF]
Oliver Szasz
Open Journal of Biophysics (OJBIPHY) , 2013, DOI: 10.4236/ojbiphy.2013.34030

Hyperthermia was the very first oncotherapy in human medicine, but its applicability in modern oncology was dubious. The discovery of electromagnetism gave new hope a century ago, however, until up to now, it has been suffering from lack of wide acceptance. Oncological hyperthermia suffers from multiple unsolved medical and technical problems. The accurate selection of malignant tissue and its proper heating in depth are real challenges together with the control and repeatability of the treatments. However, the center of the problems is not technical: the living system tries to keep its homeostatic equilibrium and creates active feedback mechanisms to eliminate or at least correct the constrain heating in depth. The proper reaction on the “gage of battle” has to involve the physiology, handle it complexly together with bioelectromagnetism and update connected technology. The solution has to be the integration of the natural bio-effects into the technological constrains, acting in synergy with the physiological feedback mechanisms, and without forcing effects out of the homeostatic control. The solution lies in strict selection and adequate action in nanoscopic range, without exciting the robust transport-mechanisms to operate against the energy delivery to the tumor. Together with the local

Innovation Clusters and Public Policy—The Case of a Research-Driven Cluster in Germany  [PDF]
Oliver Mauroner
American Journal of Industrial and Business Management (AJIBM) , 2015, DOI: 10.4236/ajibm.2015.512072
Abstract: Regional innovation clusters are spatial concentrations of interconnected firms, suppliers, service providers, state institutions, and research organisation in a particular field of innovation. The stimulation and support of clusters are important agendas for governments and other public actors. Cluster development initiatives are actually an important direction in economic policy, building on earlier efforts in macroeconomic stabilization, privatisation, and market opening, and reducing the costs of doing business. The purpose of this paper is to look on a specific type of innovative clusters in Germany, which are supported by the Fraunhofer Society, one of the leading, partly public-funded organisations for application-oriented research in Europe. Based on an overview over current issues in cluster literature—beginning with Porter 1990 to the point of actual global-value-chain-approach and the concept of knowledge hubs—the particular cluster approach of the German Fraunhofer Society is classified with regard to the academic literature. Fraunhofer clusters are, in the first instance, project clusters compared to simple communication networks. The case study presented in this paper is a valid example for a long-term and well-established industry cluster, which actually opens out in a project-oriented cluster approach. Finally, it is possible to draw practical implications for policy makers and industry regarding the support of regional innovation clusters.
Large-Scale Phenotyping of an Accurate Genetic Mouse Model of JNCL Identifies Novel Early Pathology Outside the Central Nervous System
John F. Staropoli, Larissa Haliw, Sunita Biswas, Lillian Garrett, Sabine M. H?lter, Lore Becker, Sergej Skosyrski, Patricia Da Silva-Buttkus, Julia Calzada-Wack, Frauke Neff, Birgit Rathkolb, Jan Rozman, Anja Schrewe, Thure Adler, Oliver Puk, Minxuan Sun, Jack Favor, Ildikó Racz, Raffi Bekeredjian, Dirk H. Busch, Jochen Graw, Martin Klingenspor, Thomas Klopstock, Eckhard Wolf, Wolfgang Wurst, Andreas Zimmer, Edith Lopez, Hayat Harati, Eric Hill, Daniela S. Krause, Jolene Guide, Ella Dragileva, Evan Gale, Vanessa C. Wheeler, Rose-Mary Boustany, Diane E. Brown, Sylvie Breton, Klaus Ruether, Valérie Gailus-Durner, Helmut Fuchs, Martin Hrabě de Angelis, Susan L. Cotman
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0038310
Abstract: Cln3Δex7/8 mice harbor the most common genetic defect causing juvenile neuronal ceroid lipofuscinosis (JNCL), an autosomal recessive disease involving seizures, visual, motor and cognitive decline, and premature death. Here, to more thoroughly investigate the manifestations of the common JNCL mutation, we performed a broad phenotyping study of Cln3Δex7/8 mice. Homozygous Cln3Δex7/8 mice, congenic on a C57BL/6N background, displayed subtle deficits in sensory and motor tasks at 10–14 weeks of age. Homozygous Cln3Δex7/8 mice also displayed electroretinographic changes reflecting cone function deficits past 5 months of age and a progressive decline of retinal post-receptoral function. Metabolic analysis revealed increases in rectal body temperature and minimum oxygen consumption in 12–13 week old homozygous Cln3Δex7/8mice, which were also seen to a lesser extent in heterozygous Cln3Δex7/8 mice. Heart weight was slightly increased at 20 weeks of age, but no significant differences were observed in cardiac function in young adults. In a comprehensive blood analysis at 15–16 weeks of age, serum ferritin concentrations, mean corpuscular volume of red blood cells (MCV), and reticulocyte counts were reproducibly increased in homozygous Cln3Δex7/8 mice, and male homozygotes had a relative T-cell deficiency, suggesting alterations in hematopoiesis. Finally, consistent with findings in JNCL patients, vacuolated peripheral blood lymphocytes were observed in homozygous Cln3Δex7/8 neonates, and to a greater extent in older animals. Early onset, severe vacuolation in clear cells of the epididymis of male homozygous Cln3Δex7/8 mice was also observed. These data highlight additional organ systems in which to study CLN3 function, and early phenotypes have been established in homozygous Cln3Δex7/8 mice that merit further study for JNCL biomarker development.
Atheism and Humanism in a Globalized World: The Igbo Experience  [PDF]
Chizaram Onyekwere, Oliver Uche
Open Journal of Philosophy (OJPP) , 2013, DOI: 10.4236/ojpp.2013.31A015

Obnoxious labels are derogatory terms which speak extensively on the ignorant dispositions of scholars who either rush into faulty conclusions, or have prior decisions to promote class distinction through the uncomplimentary colours they paint of what others hold as divine, spiritual, and transcendental. For such derogatory terms to gain wide audience in a globalized age explain the frame of mind of discordant voices which have been based on arm-chair scholarship. The thrust of this article therefore, is to use Igbo experience to explore the problems of atheism and humanism in a globalized world. The exploratory research will help adopt cultural centred approach in analyzing the dichotomy between the various philosophical view points on God, spirits and man’s religious belief system in Igbo land in particular and Africa in general. It is hoped that the analyses of the challenges posed by atheism and humanism in a globalized world will balance ideas, views, attitudes and behaviour that will reposition Igbo religious beliefs, values and practices in line with the proposed theistic humanism associated with Igbo culture in particular and African culture in general. This will breach the persisted conflict between the sacred and the secular pointing to a dynamic and progressive Igbo culture.


Copyright © 2008-2017 jourlib.org. All rights reserved.