The aim of the study was to determine the effect of single whole-body cryotherapy (WBC) session applied prior to submaximal exercise on the activity of antioxidant enzymes, the concentration of lipid peroxidation products, total oxidative status, and the level of cytokines in blood of volleyball players. The study group consisted of 18 male professional volleyball players, who were subjected to extremely cold air ( ) prior to exercise performed on cycloergometer. Blood samples were taken five times: before WBC, after WBC procedure, after exercise preceded by cryotherapy (WBC exercise), and before and after exercise without WBC (control exercise). The activity of catalase statistically significantly increased after control exercise. Moreover, the activity of catalase and superoxide dismutase was lower after WBC exercise than after control exercise ( ). After WBC exercise, the level of IL-6 and IL-1 was also lower ( ) than after control exercise. The obtained results may suggest that cryotherapy prior to exercise may have some antioxidant and anti-inflammatory properties. The relations between the level of studied oxidative stress and inflammatory markers may testify to the contribution of reactive oxygen species in cytokines release into the blood system in response to exercise and WBC. 1. Introduction The physical effort is a complex systemic process, which may have many health benefits including a lowered threat of all-cause mortality along with a reduced risk of respiratory and cardiovascular disease, cancer, diabetes, and many others [1, 2]. However, it is also clear that high training volume and insufficient recovery may induce muscle damage with subsequent inflammation indicated by soreness, swelling, and loss of muscle function . In modern sport, the competitive athletes are often exposed to high-intensity exercise performed multiple times per week, which may lead to overreaching and overtraining and diminish competitive performance . The short period of recovery available for the athletes requires applying the additional methods that prevent overtraining . Hence, in recent years, the scientific interest in sport medicine has been increasing in recovery modalities. A modern method used to maximize the performance of the athletes and to prevent traumas seems to be stimulation cryotherapy (cryostimulation). Initially, it was predominantly used in treating several diseases; however, its beneficial effects in postexercise recovery make it more and more often applied in sportsmen [6, 7]. For professional sportsmen, the preferred form of
S. N. Blair, Y. Cheng, and J. S. Holder, “Is physical activity or physical fitness more important in defining health benefits?” Medicine and Science in Sports and Exercise, vol. 33, no. 6, pp. S379–S399, 2001.
C. J. Crespo, M. R. Garcia Palmieri, R. P. Perdomo et al., “The relationship of physical activity and body weight with all-cause mortality: results from the Puerto Rico Heart Health Program,” Annals of Epidemiology, vol. 12, no. 8, pp. 543–552, 2002.
H. Pournot, F. Bieuzen, J. Louis, J.-R. Fillard, E. Barbiche, and C. Hausswirth, “Time-Course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise,” PLoS ONE, vol. 6, no. 7, article e22748, 2011.
R. Meeusen, M. Duclos, M. Gleeson, G. Rietjens, J. Steinacker, and A. Urhausen, “Prevention, diagnosis and treatment of the Overtraining Syndrome,” European Journal of Sport Science, vol. 6, no. 1, pp. 1–14, 2006.
G. Banfi, G. Melegati, A. Barassi et al., “Effects of whole-body cryotherapy on serum mediators of inflammation and serum muscle enzymes in athletes,” Journal of Thermal Biology, vol. 34, no. 2, pp. 55–59, 2009.
A. Wo？niak, B. Wo？niak, G. Drewa, C. Mila-Kierzenkowska, and A. Rakowski, “The effect of whole-body cryostimulation on lysosomal enzyme activity in kayakers during training,” European Journal of Applied Physiology, vol. 100, no. 2, pp. 137–142, 2007.
Z. Zagrobelny, B. Halawa, M. Negrusz-Kawecka et al., “Hormonal and hemodynamic changes caused by whole body cooling in patients with rheumatoid arthritis,” Polskie Archiwum Medycyny Wewnetrznej, vol. 87, no. 1, pp. 34–40, 1992.
J. Lepp？luoto, T. Westerlund, P. Huttunen et al., “Effects of long-term whole-body cold exposures on plasma concentrations of ACTH, beta-endorphin, cortisol, catecholamines and cytokines in healthy females,” Scandinavian Journal of Clinical and Laboratory Investigation, vol. 68, no. 2, pp. 145–153, 2008.
S. F. Nadler, K. Weingand, and R. J. Kruse, “The physiologic basis and clinical applications of cryotherapy and thermotherapy for the pain practitioner,” Pain Physician, vol. 7, no. 3, pp. 395–399, 2004.
C. Mila-Kierzenkowska, A. Wo？niak, B. Wo？niak et al., “Whole-body cryostimulation in kayaker women: a study of the effect of cryogenic temperatures on oxidative stress after the exercise,” Journal of Sports Medicine and Physical Fitness, vol. 49, no. 2, pp. 201–207, 2009.
C. Malm, P. Nyberg, M. Engstr？m et al., “Immunological changes in human skeletal muscle and blood after eccentric exercise and multiple biopsies,” Journal of Physiology, vol. 529, no. 1, pp. 243–262, 2000.
O. Sergent, I. Morel, P. Cogrel et al., “Simultaneous measurements of conjugated dienes and free malondialdehyde, used as a micromethod for the evaluation of lipid peroxidation in rat hepatocyte cultures,” Chemistry and Physics of Lipids, vol. 65, no. 2, pp. 133–139, 1993.
H. P. Misra and I. Fridovich, “The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase,” Journal of Biological Chemistry, vol. 247, no. 10, pp. 3170–3175, 1972.
D. E. Paglia and W. N. Valentine, “Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase,” The Journal of Laboratory and Clinical Medicine, vol. 70, no. 1, pp. 158–169, 1967.
E. Marin, O. H？nninen, D. Müller, and W. Klinger, “Influence of acute physical exercise on glutathione and lipid peroxides in blood of rat and man,” Acta Physiologica Hungarica, vol. 76, no. 1, pp. 71–76, 1990.
T. Inayama, Y. Kumagai, M. Sakane, M. Saito, and M. Matsuda, “Plasma protein-bound sulfhydryl group oxidation in humans following a full marathon race,” Life Sciences, vol. 59, no. 7, pp. 573–578, 1996.
B. Dawson, G. J. Henry, C. Goodman et al., “Effect of vitamin C and E supplementation on biochemical and ultrastructural indices of muscle damage after a 21 km run,” International Journal of Sports Medicine, vol. 23, no. 1, pp. 10–15, 2002.
M. Inal, F. Akyüz, A. Turgut, and W. M. Getsfrid, “Effect of aerobic and anaerobic metabolism on free radical generation swimmers,” Medicine and Science in Sports and Exercise, vol. 33, no. 4, pp. 564–567, 2001.
A. Knebel, H. J. Rahmsdorf, A. Ullrich, and P. Herrlich, “Dephosphorylation of receptor tyrosine kinases as target of regulation by radiation, oxidants or alkylating agents,” EMBO Journal, vol. 15, no. 19, pp. 5314–5325, 1996.
R. Laskowski, E. Ziemann, R. Olek, and A. Zembron-Lacny, “The effect of three days of judo training sessions on the inflammatory response and oxidative stress markers,” Journal of Human Kinetics, vol. 30, no. 1, pp. 65–73, 2011.
A. Zembron-Lacny, M. Naczk, M. Gajewski et al., “Changes of muscle-derived cytokines in relation to thiol redox status and reactive oxygen and nitrogen species,” Physiological Research, vol. 59, no. 6, pp. 945–951, 2010.
I. Kosmidou, T. Vassilakopoulos, A. Xagorari, S. Zakynthinos, A. Papapetropoulos, and C. Roussos, “Production of interleukin-6 by skeletal myotubes: role of reactive oxygen species,” American Journal of Respiratory Cell and Molecular Biology, vol. 26, no. 5, pp. 587–593, 2002.
A. Lubkowska, B. Dolegowska, Z. Szygula, and A. Klimek, “Activity of selected enzymes in erythrocytes and level of plasma antioxidants in response to single whole-body cryostimulation in humans,” Scandinavian Journal of Clinical and Laboratory Investigation, vol. 69, no. 3, pp. 387–394, 2009.
A. Wozniak, B. Wozniak, G. Drewa, and C. Mila-Kierzenkowska, “The effect of whole-body cryostimulation on the prooxidant—antioxidant balance in blood of elite kayakers after training,” European Journal of Applied Physiology, vol. 101, no. 5, pp. 533–537, 2007.
B. Dugué, J. Smolander, T. Westerlund et al., “Acute and long-term effects of winter swimming and whole-body cryotherapy on plasma antioxidative capacity in healthy women,” Scandinavian Journal of Clinical and Laboratory Investigation, vol. 65, no. 5, pp. 395–402, 2005.
E. Miller, ？. Markiewicz, J. Saluk, and I. Majsterek, “Effect of short-term cryostimulation on antioxidative status and its clinical applications in humans,” European Journal of Applied Physiology, vol. 112, pp. 1645–1652, 2012.
S. G. Rhind, J. W. Castellani, I. K. M. Brenner et al., “Intracellular monocyte and serum cytokine expression is modulated by exhausting exercise and cold exposure,” American Journal of Physiology, vol. 281, no. 1, pp. R66–R75, 2001.
E. Ziemann, R. A. Olek, S. Kujach, et al., “Five day whole-body cryostimulation, blood inflammatory markers, and performance in high-ranking professional tennis players,” Journal of Athletic Training, vol. 47, no. 6, pp. 664–672, 2012.
A. Lubkowska, Z. Szygu？a, D. Chlubek, and G. Banfi, “The effect of prolonged whole-body cryostimulation treatment with different amounts of sessions on chosen pro-and anti-inflammatory cytokines levels in healthy men,” Scandinavian Journal of Clinical and Laboratory Investigation, vol. 71, no. 5, pp. 419–425, 2011.
S. Guo, W. Wharton, P. Moseley, and H. Shi, “Heat shock protein 70 regulates cellular redox status by modulating glutathione-related enzyme activities,” Cell Stress and Chaperones, vol. 12, no. 3, pp. 245–254, 2007.
A. Wyttenbach, O. Sauvageot, J. Carmichael, C. Diaz-Latoud, A.-P. Arrigo, and D. C. Rubinsztein, “Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin,” Human Molecular Genetics, vol. 11, no. 9, pp. 1137–1151, 2002.
A. Y.-C. Liu, H. Bian, L. E. Huangi, and Y. K. Lee, “Transient cold shock induces the heat shock response upon recovery at 37°C in human cells,” Journal of Biological Chemistry, vol. 269, no. 20, pp. 14768–14775, 1994.
E. Ziemann, A. Zembroń-？acny, A. Kasperska, et al., “Exercise training-induced changes in inflammatory mediators and heat shock proteins in young tennis players,” Journal of Sport Science and Medicine, vol. 12, pp. 282–289, 2013.
K. D. Fairchild, R. M. Viscardi, L. Hester, I. S. Singh, and J. D. Hasday, “Effects of hypothermia and hyperthermia on cytokine production by cultured human mononuclear phagocytes from adults and newborns,” Journal of Interferon and Cytokine Research, vol. 20, no. 12, pp. 1049–1055, 2000.
J. W. Castellani, I. Brenner, and S. Rhind, “Cold exposure: human immune responses and intracellular cytokine expression,” Medicine and Science in Sports and Exercise, vol. 34, no. 12, pp. 2013–2020, 2002.